Showing posts with label Forensic Medicine. Show all posts
Showing posts with label Forensic Medicine. Show all posts

Wednesday, April 15, 2009

- Blood groups - ABO system.

- Many systems have been discovered to differnetiate bloog groups,the most famous system is ABO system.




- ABO system represent antigenic components on the surface of RBCs according to which
this system was made.
- O RBCS means that there is no antigens on the surface of RBCs so we can give O RBCS
to all other types of RBCs groups.

- B RBCS means that there is B antigens on the surface of RBCs so we can give B RBCS
Only to B person or AB person.

- A RBCS means that there is A antigens on the surface of RBCs so we can give A RBCS
Only to A person or AB person.

- AB RBCS means that there Are both A,B antigens on the surface of RBCs so we can give AB RBCS Only to AB person.

Thursday, March 26, 2009

-Sudden Infant Death Syndrome.

Sudden Infant Death Syndrome.
(SIDS) Or COT death



Def:- Sudden unexpectrd death in infant of appearant good health,without giving a commonly accepted cause at autopsy.

The cause:- undefinite but may be:-
  1. Suffocation.
  2. Infections:fulminant viral infections.
  3. Impaired respiratory reflexes.
  4. Diet : Improper diet causing nutritional deficiences or hypernatremia.
  5. Sensetivity to milk proteins.
  6. Cardiac conduction disorders.
  7. Co or Co2 poisoning.
  8. Overlying in the early life.
  9. Thermal: Hyper or hypothermia.

*
Features of the syndrome:

- Age: () 2weeks and 2 years with a peak 2-3 months of life.

- Sex : males more than  females.

- Social level: low socioeconomic level more than high socioeconomic level.

- Whether: cold winter more than hot summer.

- Twins more than single babies due to:

  •   increase icidence prematurity.
  •   low birth weight.

Sunday, January 25, 2009

- Some Types of prints.

- Really there are many types of prints which help

in identification of individuals allover the world,

especially in the field of forensic medicine and crimes.

- We will take a quick review about some of them:

1- Fingerprints..from here.

2-DNA fingerprinting...from here.

3-Foot prints:

- are the impressions or images left behind by a

person walking made by sole of the foot and


skin pattern of toes and heel.

- WE can collect important information from them

that helps tell about the person that left them behind.

-Information concerning the approximate height of the

person and possibly the walking habits of the person

are two pieces of information that can prove useful

in a criminal investigation.

4-palm prints.

5-Iris print (recognition):

-Are used to screen individuals who are trying to gain

access to more highly secure places or accounts,

not to scan the general public at random.

-By using camera technology, with subtle infrared

illumination reducing specular reflection from

the convex cornea, to create images of the detail-rich,

intricate structures of the iris. Converted into

digital templates, these images provide

mathematical representations of the iris that yield

unambiguous positive identification of an individual.

6-Retinal print:the same idea of iris.

6-Voice print:

-The fact that voice follows a particular pattern,

whatever the language spoken.

- The FBI is trying to develop a system that

could make your voice as unique and recognizable

as your fingerprint called FASR system.


7-Ear print:

Burglars often listen at windows and doors,

leaving an earprint behind, which, just like

a finger print, can be used to trace them.

Ears are unique in size, shape and structure.

Scientists use these traits to develop biometric

scans of the ear. In ear scans, a camera creates

an image of the ear that is analyzed for

identifying characteristics.

8-Thermal footprint:

our bodies emit thermal energy which by special systems

can recognize the persons after their presence

in some places e.g scene if the crimes.


9- Air sinus print:

Pattern of skull's frontal air sinuses ,Outline is

unique and comparisons with antemortem X-rays are useful.

10-Lip print:-

lip print identification is generally acceptable

within the forensic science as a means of positive

identification because it appears in the scene of the crime.


see you, Dr Ibrahim

Saturday, January 17, 2009

- Sir Hershel -1858 .



Sir William Herschel, Chief Magistrate of the Hooghly

district in Jungipoor, India, first used fingerprints on

native contracts.

On a whim, and with no thought toward personal

identification, Herschel had Rajyadhar Konai, a local

businessman, impress his hand print on a contract.




The idea was merely "... to frighten [him] out of

all thought of repudiating his signature." The native was

suitably impressed,

and Herschel made a habit of requiring palm prints

--and later, simply the prints of the right Index and

Middle fingers--on every contract made with the locals.

Personal contact with the document,

they believed, made the contract more

binding than if they simply signed it.

Thus, the first wide-scale, modern-day use

of fingerprints was predicated,

not upon scientific evidence,

but upon superstitious beliefs.

As his fingerprint collection grew,

however, Herschel began

to note that the inked impressions could,

indeed, prove or disprove identity.

While his experience with fingerprinting was

admittedly limited, Sir Herschel's private

conviction that all fingerprints were

unique to the individual, as well as permanent

throughout that individual's life,

inspired him to expand their use.

Source here....

Monday, January 12, 2009

- DNA fingerprinting.


- The chemical structure of everyone's DNA is the same.

- The only difference between people (or any animal)

is the order of the base pairs.

- There are so many millions of base pairs in each

person's DNA that every person has a different sequence.

-There are high diversity due to presence of non encoding

areas in the strand either in the genetic area

or outside it see here...

- Using these sequences, every person could

be identified by the sequence of their base pairs.

- Each person has a unique DNA fingerprint,

except monozygous (identical) twins.

- DNA fingerprint is the same for every cell,

tissue, and organ of a person. It cannot be

altered by any known treatment. Consequently,

DNA fingerprinting is rapidly becoming the primary

method for identifying and distinguishing among

individual human beings.

- These patterns do not, however, give an

individual "fingerprint," but they are able to

determine whether two DNA samples are from the

same person, related people, or non-related people.

Scientists use a small number of sequences of DNA

that are known to vary among individuals ,

and analyze those to get a certain probability of a matching.

------------------------------------------------

**Practical Applications of DNA Fingerprinting**



1. Paternity and Maternity :

when a father deny that a certain child is belonging

to him Or tow sets of parents claim on one child.

2. Criminal Identification and Forensics:

- In living (strong evidence of involvement

in assault,rape, disputed paternity)

as DNA isolated from blood,hair, skin cells,

or other genetic evidence left at the scene

of a crime can be compared with the

DNA of a criminal suspect .

- In Dead (DNA survives in bone for many years,

comparison of DNA with family members)

3. Personal Identification .

Enjoy it,see you,

Ibrahim

Sunday, January 11, 2009

- DNA strucure and terminology.

Chromosomes are thread-like structures located inside the nucleus of cells. Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure.

see the picture which illustrate that also show the true genetic content & non genetic areas....

Promoters: are DNA sequences adjacent to the beginning of the genes and control gene activation.
 
Introns are sequences inside the gene although they do not code for protein sequence they are necessary for correct coding.
 
Exons are the regions inside the gene between introns that contain the coding DNA sequences. 
 
Most of the harmful mutations occur in exons and as a consequence change the structure of the coded protein.
 

Friday, January 9, 2009

- Fingerprints.



-friction skin (i.e. palms and soles) have a

impressions due to presence of ridges & grooves .

- These impressions made by dermal papillae in the dermis.

- Finger prints appear in the 4th month intrauterine life.

-Sweat glands open through minute openings on the

summits of the ridges. The sweat contains fat.

- When the skin is applied to a glistening

non-absorbable
surface an impression is left behind.


Principles of fingerprint identification :

-fingerprint patterns are unique

(1 in 64 billion chance of 2 prints being identical).

-FBI has over 100 million records, no two of which are alike.

-Fingerprint pattern of an individual remains

unchanged throughout life.

-Reversible atrophy occurs in certain diseases

(coeliac disease, dermatitis).

- Some people have some skin diseases which

prevent normal formation

of fingerprints (may be genetically ).

-Permanent impairment occurs in leprosy and after

exposure to radiation.

-Attempts to mutilate fingerprints are sometimes made.

- If only the epidermis is destroyed there is no

alteration in ridge pattern.

-If dermis is destroyed additional points of

identification are created.

Types:

1-Arches.

2-Loops.

3-Whorls .

4-composite: of more than one type
.


-----------------------------
poroscopy
Def: study of the pores of the sweat glands which
present at the ridges of the prints.


Position of the pores requires higher resolution scanner.

---------------
N.B: There are artificial finger prints which
used by criminals for misleading.

see you,
Dr Ibrahim

Sunday, January 4, 2009

- IDENTIFICATION (4).

IDENTIFICATION OF THE DEAD

Through:
  • Primary Physical Characteristics
  • Secondary Physical Characteristics
  • IDENTITY of DECOMPOSED or SKELETAL REMAINS
1- Primary Physical Characteristics:

- characteristics which are very difficult for a person to change during life.
Some of these characteristics will appear to alter postmortem:

-Sex.
-Age :- external appearances, internal degenerative disease, bones, joints.
-Height or stature :-
N.B. height of Body differs from that in life by up to 2-3 cm, due to joint and muscle relaxation.

-Weight :- Body often appears of different build to that in life
-Race.
-DNA (unique to every individual.)
-Fingerprints

2- Secondary Physical Characteristics:

-characteristics which can change during life,either deliberately by deceased or as a result
of medical/dental interference.
-Some of these characteristics will appear to alter postmortem:-

-Skin colour (alters post mortem).
-Eyes :- more useful in caucasians than negroid & mongoloid races.
Colour can alter PM
-Teeth :- very resistant and bear much useful information.
-Hair :- colour, style, length, beard/moustache.
-Scars :- surgical procedures and prostheses.

-Tattooing :- seen even if the body is putrefied.

-External peculiarities :- circumcision, moles, warts.
-Deformities.
-Clothing and other objects as Jewelery,Cosmetics.


-------------------------------------------

IDENTIFICATION OF THE LIVING

METHODS OF IDENTIFICATION:

The above of dead +

1-Personal impression (visual identification):

Personal impression depends on features such as

hair, moustache and beard,any gait particularities,

voice characters or hand movement during speech .

2-Photography.

3-Handwriting:

-Possible for experts to identify a person .
-we can know either the person left or right handed.
-Methods used include :
  • photographic enlargement,
  • analysis of ink,
  • analysis of paper.
see identification (1) here...

see identification (2) here...

see identification (3) here...



Thank you for attention,


"The end of IDENTIFICATION"

Dr Ibrahim

Saturday, January 3, 2009

- IDENTIFICATION (3 ).

DETERMINATION OF STATURE FROM BONES

- Long bone length (femur, humerus) is proportional to height.

- There are sex, race, nutrition and personal variations to consider

- By karl pearson formula.

Humerus = 18% of stature
Femur = 25% of stature

-----------------------------

Determination of postmortem interval from bone.

- postmortem interval (PMI) : period () death & examination.

- Are they ancient or modern bones?

(i.e. greater or less than 50 years )

-Rate of skeletonisation is highly variable.

  • In the tropics a body can be reduced to a skeleton in 3 weeks.
  • Remarkable preservation of body is seen in acidic peaty soil
(e.g. "Pete Bogg" from Cheshire was 200 years old!).

Thus, environmental conditions have to be taken into account.

-Naked eye appearance is unreliable:
  • Tags of soft tissue, periosteum, ligaments etc, indicate less than 5 years old.
  • Soapy texture of surface indicates age less than a few decades.
  • Light, crumbling bones are likely to be a century or more old.
---------------------------------------
Individualizing skeletal features
  • Bone disease (Paget's disease, tumours) Previous injury to bone (fracture callus, prosthesis, metallic fragments).
  • Comparison of trabecular pattern of bone.
  • Pattern of skull's frontal air sinuses ,Outline is unique and comparisons with antemortem X-rays are useful.
- Facial reconstruction:-
  • Skull can be scanned into a computer and "fleshed" by computer reconstruction to give likely facial appearance in life.
  • Unfortunately eye colour, hair colour and lips are independent of bony structure.

- Laboratory tests –

1.precipitin test :
Immunological reaction between bone extract and anti human serum ceases within months of death.

2.If blood pigments are present bones are usually less than 10 years old.

3.Up to 20 amino acids may be identified in bones less than a century old.

4.Fluorescence of freshly sawn bone surface under UV light diminishes after 100 years.

5.New bones contain 4.0 - 4.5 gms% nitrogen; 2.5 gms% indicates approximately 350 years.

6.Radioactive carbon dating indicates which century.

see identification (1) here...

see identification (2) here...

see identification (4) here...

Tuesday, December 23, 2008

- Identification (2).

4•Age estimation:

A- from skull :

- dimensions:-

-eg , at full term
  • circumference =13 inches
  • length =5 inches
  • width = 4 inches



- fontanelles closure :-
  • Anterior fontanelle close at 2 years.
  • posterior fontanelle close at 9 months (full term).
- suture fusion.


B- from union of the epiphysis:

- Sex has to be taken into account as;bone development and epiphyseal
fusion is
different between the sexes.

- The pattern of fusion of bone ends (epiphysis) to bone shaft (metaphysis) in each bone indicates age.

C- from ossific centers of the bones.

--------------------------------

5•RACE DETERMINATION:

- The skull is the only reliable bone.

  • Caucasian (all whites)
  • Negro (all blacks - African, American, Negroes and West Indians)
  • Mongoloid (Chinese, Japanese, American Indians)

- Thus skulls of British, Germans, French or
Swedes cannot be distinguished from one another.
- Similarly Japanese skulls are similar to Chinese skulls.


see identification (1) here...

see identification (3) here...

see identification (4) here...

Monday, December 22, 2008

- Identification (1).

Identification( الإستعراف)

-Def:- recognition of a person (living or dead) through certain characters that
differentiate him from all other persons.


-Include :-
  • Skeletal remains( Bony collection),
  • Identification of the living person,
  • Identification of the dead body or part of dead body.
-Identification of Skeletal remains( Bony collection)

1•Are the remains human or animal?

- If complete bone....by anatomical features.

- If piece of bone (where there is no anatomical
features) do Precipitin test.

-N.B:- Precipitin test:-
  • Crushing a small piece of bone & suspending it in saline.
  • The saline extraction is examined against human antiserum.
  • This test not working with burned bone due to destruction of proteins.



2•How many bodies? ( Number of persons)..

Through:

- Repetition of the same single bones

e.g (2 skulls,2 mandibles,2 sternii..etc).

- Different sex ,age,race,Postmortem interval.

3•sex differentiation:

A) From height of long bone.

B) Based on appearance of :-

Skull,Mandible,Sternum, Pelvis,Hip bone,sacrum.


Before puberty:- It is impossible to differentiate

the sex from bone.As sexual characters aren't manifested yet.

After puberty:-It is possible to differentiate the sex from bone.

A) From height of long bone.

  • By the height of the bone we can suggest sex.
  • By using Karl Pearson's formula: -
Femur= 25% of height.
Humerus = 18% of height.

-Skull :




-Mandible:-

-Sternum:-

- Hip bone :-



- sacrum :




see identification (2) here...

see identification (3) here...

see identification (4) here...

Monday, October 20, 2008

- Forensic Medicine.


- Forensic Science :-
Application of knowledge of science (biology, chemistry, physics ,….etc) for  purposes of law and justice.(1)

- Forensic medicine (legal medicine) :-
 Is a branch of medicine that applies the principles and knowledge of
 the medical sciences for administration of law and  justice .(1)

- Forensic medicine is a medical science that studies :-
  • Identification الإستعراف
  • Somatic and Psychiatric injuries,
  • Tanatology “ science of Death. ”
  • Autopsy.
  • Medical ethics أخلاق المهنة
  • Forensic laboratory services)e.g Toxicology, Histopathology, Serology, Biochemistry, Molecular Biology, DNA laboratory, Medical Imagination and Radiology,Microbiology, Physical Technology)
- Forensic medical expert have to know medical sciences very well to use that in his final medical decisions.

- Characteristics of forensic medical expert:-
1- Caution; He must be accurate and precise in his result and decisions.
2- Intelligence; He must be a good observant.
3-Honesty and accuracy.
4-Good medical knowledge of all medical branches.
5- Well knowledge of criminal sciences and criminal law.
- The expert witness differs from the ordinary witness as he did not see or hear the incident in dispute (النزاع), he gives evidence of scientific fact and he gives an opinion based on professional knowledge and experience, though he may subsequently see the body, or the vehicle, or the instrument, or the scene.(2)


- The expert should explain that his evidences have scientific limitation , so the expert should be under an obligation(ملزم) to make sure that the court does not, unwittingly, use his evidence without realizing its scientific limitations.


- N.B:-The first ever medico-legal expert is considered to be “Imhotep” (1)


-Branches of forensic medical centre:-
  • Clinics:-
to examine the somatic and psychiatric injuries, and to determinate the age of live people through clinical forensic & forensic psychiatric .
  • Morgue(مشرحة) :- to perform autopsy.
  • Laboratories:-
-Forensic Pathology:- To study histopathology samples.
-Forensic Medical Imagination and Radiology.
-Forensic Toxicological:-To investigate the poisons, and drugs of human samples.
-Forensic Immunological :- To investigate the biological traces ( blood, semen, saliva, hair,…etc) To determine the -blood system groups, and DNA fingerprint.
-Forensic Physical Technology :- To reconstruct the accidents, the crimes, the face features, the body features, traffic accidents, firearms, and falls from heights….. to study the fingerprints.

-References:-
  • Forensic medicine ,Prof.Dr.Abd El-Aziz Ghanem,Mansoura, pp.(i-iii),(2.
  • Expert Forensic Evidence, Alec Samuels, Medicine, Science and the Law, 1974,volume 14, pp. 17-25.